Large scale prep

Our large-scale prep service is ideal for experiments requiring high-purity plasmid DNA. We use validated purification kits to provide plasmid DNA with concentration and purity (A260/280 1.8~2.0) measurements and partial sequence analysis results. For specific plasmids where recombination may lead to sequence los (e.g., Lentiviral, Retroviral, AAV), we use specialized E. coli strains to minimize recombination risks.

Applications

- Mammalian cell transfection
- Animal experiments
- Other experiments requiring endotoxin-free plasmid

Experimental
Workflow

  • STEP1

    Order Request
    - Consultation
    - Order Processing

  • STEP2

    Sample Collection
    - Plasmid

  • STEP3

    Amplification
    - Transformation
    - Seed Culture
    - Main Culture

  • STEP4

    Purification
    - MIDI Prep , - MAXI Prep
    - MEGA Prep
    - GIGA Prep , - QC

  • STEP5

    QC Data and Delivery
    - Cloned DNA , - QC Data
    - Sequencing Raw Data

Order Information - Service Pricing

Service Type Copy Number Culture Volume Guaranteed Amount Endotoxin Free Price (Excluding VAT)
MIDI LOW Copy 400 ml No guarantee NO 120,000 KRW
High Copy 200 ml 100 µg
MAXI LOW Copy 600 ml No guarantee NO 300,000 KRW
High Copy 400 ml 300 µg
MEGA LOW Copy 2 L No guarantee YES 700,000 KRW
High Copy 500 ml 1 mg
MIDI LOW Copy 3 L No guarantee YES 1,000,000 KRW
High Copy 2 L 2 mg

* GIGA Prep is processed using an Endotoxin Free kit; however, endotoxin QC is not provided.

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